After entering the body, viruses proliferate in cells, infect livestock and crops, and cause widespread damage. Based on the idea that viral infection can be prevented if we can stop viruses from growing, we developed two novel virus inactivation methods, both of which use artificial nucleic-acid-binding proteins as a key molecule to recognize viral genome. First technology is inhibition of virus replication protein function by blocking its binding to a viral replication origin with our artificial DNA-binding protein. Second technology is inhibition of virus replication by cleaving viral genome with our artificial restriction enzyme. In this project, we try to further develop these technologies to solve issues in livestock and agricultural crops. [BRAIN (NARO) Integration research for agriculture and interdisciplinary fields]